For statistical evaluation of gene expression data, AGD, NR, body weights and

organweights,one-wayANOVAwasemployedforallgroups.Dunnett’stestwasper-

formed to determine differences between treated and control group means. When

more than one pup from each litter was examined, statistical analysis was adjusted

usinglitterasanindependent,randomandnestedfactorinANOVA.Whenwepooled

data from both studies (1+2), ‘study’ was included in the statistical analysis as a

dichotomous factor. Pooled data are only reported if no significant effect of ‘study’

and no interaction between ‘study’ and ‘dose’ were observed. Data analysis of AGD

included the cube root of body weight as a covariate in the analysis, to correct for the

relationship between body size and AGD. In the analysis of PND 16 organ weights,

body weight was used as a covariate. F

Christiansen et al. 2010