IgG1 antibody titer (SRBC)

Endpoint Details

Endpoint nameIgG1 antibody titer (SRBC)
Systemimmune and lymphatic system
Effectantibody (B cell) mediated immunity: functional
Effect subtypeSecondary IgG
Diagnostic (as reported)ELISA
Observation time61 days
Additional tags
Data reported?
Data extracted?
Values estimated?
Location in literatureTable 2
NOEL0.417 mg/kg-day
LOEL0.833 mg/kg-day
Monotonicityvisual appearance of monotonicity
Statistical test descriptionone-way ANOVA followed by a Dunnett's test
Trend resultnot reported
Power notes"underpowered to detect a change of 10% control" (sample size <50% of recommended) [calculated n=98; mean required-N calculation from all dose-groups] "underpowered to detect a change of 20% control" (sample size <50% of recommended) [calculated n=26; mean required-N calculation from all dose-groups]

Dataset

Dose (mg/kg-day)Number of AnimalsResponse (ng/mL)Standard Error
a NOEL (No observed effect level)
b Significantly different from control (p < 0.05)
c LOEL (Lowest observed effect level)
0648.473.65
0.008646.113.59
0.017659.074.51
0.083654.725.21
0.417a661.934.95
0.833b,c664.184.42

Plot

0.00.20.40.60.84050607080Dose = 0 mg/kg-day N = 6 Response = 48.47 ng/mL Standard Error = 3.65Dose = 0.0083 mg/kg-day N = 6 Response = 46.11 ng/mL Standard Error = 3.59Dose = 0.0167 mg/kg-day N = 6 Response = 59.07 ng/mL Standard Error = 4.51Dose = 0.0833 mg/kg-day N = 6 Response = 54.72 ng/mL Standard Error = 5.21Dose = 0.4167 mg/kg-day N = 6 Response = 61.93 ng/mL Standard Error = 4.95Dose = 0.8333 mg/kg-day N = 6 Response = 64.18 ng/mL Standard Error = 4.42*Statistically significant at 0.05Dose (mg/kg-day)Response (ng/mL)IgG1 antibody titer (SRBC)DosesLOELNOEL
Methodology
animals were injected on the 54th day of dosing and received a challenge into the right rear footpad (saline in the left) and response was measured with microcaliper and immunoglobulin levels measured; not clear if anmals also received a booster on the last day of dosing