Mecp2308/y males and wild-type C57/Bl6 females (Jackson Laboratories) were bred to produce female offspring that were Mecp2308/+. Dams were housed in groups (maximum three per cage) and placed individually in separate cages at 5 weeks of age. Beginning at 46 days of age, heterozygous Mecp2308/+ dams were housed individually and dosed orally with either vehicle control (corn oil) or 0.03 mg BDE-47/kg/day for a 28-day period. At the end of this period, they were mated with C57Bl/6J males (obtained from JAX West, West Sacramento, CA, USA). Dosing was continued throughout mating, pregnancy and lactation. This mating scheme resulted in offspring with four possible genotypes per treatment group: Mecp2+/+, Mecp2308/+, Mecp2+/y, and Mecp2308/y.

Mouse (Mecp2 308+/-)

HAWC