Wens 2013

Data type(s)In vitro
Full citationWens B, De Boever P, Verbeke M, Hollanders K, and Schoeters G. 2013. Cultured human peripheral blood mononuclear cells alter their gene expression when challenged with endocrine-disrupting chemicals. Toxicology 303: 17-24.
AbstractEndocrine disrupting chemicals (EDCs) have the potential to interfere with the hormonal system and may negatively influence human health. Microarray analysis was used in this study to investigate differential gene expression in human peripheral blood cells (PBMCs) after in vitro exposure to EDCs. PBMCs, isolated from blood samples of four male and four female healthy individuals, were exposed in vitro for 18h to either a dioxin-like polychlorinated biphenyl (PCB126, 1μM), a non-dioxin-like polychlorinated biphenyl (PCB153, 10μM), a brominated flame retardant (BDE47, 10μM), a perfluorinated alkyl acid (PFOA, 10μM) or bisphenol (BPA, 10μM). ANOVA analysis revealed a significant change in the expression of 862 genes as a result of EDC exposure. The gender of the donors did not affect gene expression. Hierarchical cluster analysis created three groups and clustered: (1) PCB126-exposed samples, (2) PCB153 and BDE47, (3) PFOA and BPA. The number of differentially expressed genes varied per compound and ranged from 60 to 192 when using fold change and multiplicity corrected p-value as filtering criteria. Exposure to PCB126 induced the AhR signaling pathway. BDE47 and PCB153 are known to disrupt thyroid metabolism and exposure influenced the expression of the nuclear receptors PPARγ and ESR2, respectively. BPA and PFOA did not induce significant changes in the expression of known nuclear receptors. Overall, each compound produced a unique gene expression signature affecting pathways and GO processes linked to metabolism and inflammation. Twenty-nine genes were significantly altered in expression under all experimental conditions. Six of these genes (HSD11B2, MMP11, ADIPOQ, CEL, DUSP9 and TUB) could be associated with obesity and metabolic syndrome. In conclusion, microarray analysis identified that PBMCs altered their gene expression response in vitro when challenged with EDCs. Our screening approach has identified a number of gene candidates that warrant further study.
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Literature review tagsMechanistic Study
COI reportedAuthors report they have no COI
Funding sourceEuropean Community's Seventh Framework Programme and a VITO fellowship
Study identifier(Wens 2013)
Author contacted?
Author contact detailsAuthor contacted in April 2015 to obtain information for RoB assessment.
Summary/extraction commentsData extracted:
  • Table 1: note list of genes upregulated and genes downregulated by PFOA
  • Table 2: note list of immune related processes (n and p-value) for PFOA (innate immune response, Type I interferon-mediated signaling pathway, Cellular response to type I interferon

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