Social recognition tests: Juvenile F3 C57Bl/6J mice were tested for social recognition using standard acceptable methods (+ for methods). The authors note that "all behavioral tests were scored by observers who were blinded to animal lineages" (++ for blinding). Overall = +

Gene expression (hypothalamus): Quantitative PCR was performed for gene expression (++ for methods). Blinding was not reported, but correspondence with the study authors on other studies indicate that outcomes were assessed blind (+ for blinding). Overall rating = +

Protein expression (amygdala): Western blotting was used for protein expression (++ for methods). Blinding was not reported, but correspondence with the study authors on other studies indicate that outcomes were assessed blind (+ for blinding). Overall rating = +

Wolstenholme et al. 2019

Risk of bias

HAWC