Estrus Cyclicity: Estrus cycle was determined via vaginal cytology, no other description of the methods or information on what constituted a regular pattern of cyclicity was not provided. (+for methods). Blinding was not reported (NR for blinding). Overall rating = NR

LH hormone levels: LH was determined via "rat LH assy kit" from the National Hormone and Pituitary Program. It was reported that the intra-assay variability was 7% (+ for methods). Blinding was not reported. Although all

samples were noted to be run on the same kit, there is no indication that samples were not individually pipetted in or randomly placed in the wells (NR for blinding). Overall rating = NR

Genital tract abnormalities: only stated that animals were examined for evidence of macroscopic abnormalities of the genital tract. What organs were included was not reported (NR for methods). Blinding was not reported (NR for blinding). Overall= NR

Rubin et al. 2001

Risk of bias

HAWC