| developmental rat |
Developmental |
A total of 40 healthy pure SD rats, equal male and
female, were purchased from Guizhou Laboratory Animal Engineering
Technology Center for the study. The selected rats were randomly divided
into two groups (control group, fluoride exposure group) with equal size based
on body weight using random number table method. The control group was given tab
water every day (containing fluoride less than 0.5 mg/L); the
fluoride exposure group was given sodium fluoride at 50 mg/L. Rats from both
groups were given standard animal food containing fluoride less than 6.2
mg/kg. After a six-month continuous dosing period, female and male rats, with a
ratio of 1:1, were mated. Offspring were fed with breastmilk. On
day 28, offspring were selected to conduct behavior tests (Morris water maze). |