| 30 days |
Subchronic (30-90 days) |
The animals were divided into six groups (n=6 in each group):
Group 1: Control-sedentary rats receiving tap water.
Group 2: Toxicated-sedentary rats exposed to 600 ppm NaF for a month through drinking water.
Groups 3-6: F-toxicated rats (600 ppm NaF fed through water for a month) were further subgrouped (groups 3-6) and subjected to intermittent swimming exercise regimen for about 45 min in a glass tank (length 100 cm, width 50 cm, and depth 50 cm) for 10 days at controlled specific temperatures 20, 25, 30, and 35 deg C.; Three-month-old male albino rats, Wistar strain (Rattus norvegicus albinus), weighing 180-220 g were procured from Sri Raghavendra Enterprises, Bangalore and acclimatized to laboratory conditions (25 +/- 2 deg C with 12 dark/light cycle) in individual standard polyethylene cages (41 x 28 x 14 cm) (B I.K Industries, Mumbai) with stainless steel top grill and were housed in groups of three per cage. During the whole experimentation, all animals had access to tap water and food pellets (Amruth feeds, India) ad libitum. |