Wang 2004a
| Data type(s) | Animal bioassay |
|---|---|
| Full citation | Wang JD, Ge YM, Ning HM, Wang SL. 2004. Effects of high fluoride and low iodine on biochemical indexes of the brain and learning-memory of offspring rats. Fluoride 37: 201-208. |
| Abstract | Thirty-two Wistar rats were divided randomly into four groups of eight rats (female:male = 3:1). With one untreated group as controls, the other three groups were administered, respectively, high fluoride in their drinking water (100 mg NaF/L), low iodine (0.0855 mg/kg chow), or both high fluoride (100 mg NaF/L) and low iodine (0.0855 mg/kg) in order to assess the effects of the above three factors on the learning and memory ability of their offspring rats. After the animal model was established, the rats were allowed to breed, and 36 offspring rats in each group (female:male = 1:1) were randomly selected for the experiments. The treatment of these rats was the same as their parents. In comparison with control rats, the learning and memory ability of the offspring rats was depressed by high fluoride, low iodine, or the combination of high fluoride and low iodine. Brain protein was decreased by low iodine and even more by the combined interaction of high fluoride and low iodine. The activity of cholinesterase (ChE) in the brain was affected to some extent by high fluoride and low iodine but was especially affected by high fluoride and low iodine together. |
| COI reported | Not reported |
| Funding source | China National Natural Science Foundation |
| Study identifier | 354 |
| Author contacted? | true |
| Author contact details | The author, Jundong Wang, at wangjd53@outlook.com was contacted about randomization, allocation concealment, exposure characterization, and statistical analyses. No response to the query has been received. |
| Summary/extraction comments | Data not extracted: body weights in offspring; ratio of brain and thyroid weight to body weight; brain protein content; brain cholinesterase (ChE) activity; endpoints measured in animals on a low iodine diet or co-treated with low iodine diet + high fluoride Originally assessed in the 2016 Fluoride assessment: https://hawcproject.org/assessment/126/ |
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Animal bioassay experiments
| Name | Type | Comments |
|---|---|---|
| Repro/Developmental | Developmental | Thirty-two of the one-month old Wistar rats albino rats (female:male = 3:1) were randomly divided into four groups of six females and two males each and were maintained on the diets and water shown in Table 1 under standard temperature (22–25 deg C), ventilation, and hygienic conditions. Three months after establishing the animal model, the female experimental animals were allowed to become pregnant by natural mating. The day of birth of the offspring rats was set as day 0. During and after nursing, they were raised under the same conditions as their parents. After one month, the offspring rats were separated according to sex. At day 0 and then at day 10, 20, 30, 60, and 90, three male and three female offspring rats were randomly selected from each litter for study.; One-month old Wister albino rats, each weighing approximately 50 g, were obtained from the Experimental Animal Center of Shanxi Medical University for use in this study… The normal diet was provided by the Experimental Animal Center of Shanxi Medical University. |