| Repro/Developmental |
Developmental |
Thirty-two of the one-month old Wistar rats albino rats (female:male = 3:1) were randomly divided into four groups of six females and two males each and were maintained on the diets and water shown in Table 1 under standard temperature (22–25 deg C), ventilation, and hygienic conditions. Three months after establishing the animal model, the female experimental animals were allowed to become pregnant by natural mating. The day of birth of the offspring rats was set as day 0. During and after nursing, they were raised under the same conditions as their parents. After one month, the offspring rats were separated according to sex. At day 0 and then at day 10, 20, 30, 60, and 90, three male and three female offspring rats were randomly selected from each litter for study.; One-month old Wister albino rats, each weighing approximately 50 g, were obtained from the Experimental Animal Center of Shanxi Medical University for use in this study… The normal diet was provided by the Experimental Animal Center of Shanxi Medical University. |