| rat NaF water |
Chronic (>90 days) |
The animal model of chronic fluorosis was established by feeding Wistar rats on distillated water containing different concentrations of sodium fluoride (control group: 0 mg/L, low fluoride exposure group: 50 mg/L, medium fluoride exposure group: 100 mg/L, and high fluoride exposure group: 150 mg/L) for six months. In addition to the water, all the rats were fed with standard animal food.; A total of forty one-month old healthy male Wister rats, weighting around 100 g, were provided by College of Veterinary Medicine Yangzhou University for the experiment. The selected rats were randomly divided into 4 groups (10 per group): control group, low fluoride exposure group, medium fluoride exposure group and high fluoride exposure group. |