Pups at PND1 were necropsied and liver tissue was removed for further analysis. The remaining pups were placed on either a high fat or control diet until PND110. Body weight, food intake, and body composition measurements were taken throughout the study. At necropsy, livers from PND110 pups were collected for histopathology.

Body weight: methods not reported, but are likely standard (+ for methods). Blinding was not reported (NR for blinding). Overall rating = NR

Cumulative Energy Intake: was calculated by weighing pellets remaining on the fourth day, subtracting the initial food supplied, and multiplying daily calculated intake by the caloric composition of each diet. This would have to have been done on groups of animals as they were housed 2-3 per cage (+). Blinding was not reported (NR)

Liver Lipid Measurements: Hepatic triglyceride levels and free fatty acid levels/composition were measured in PND1 pups. Standard methods were used for extracting lipids from liver tissue and measuring lipid content, but there was no description on QC methods or recovery rates of the method (+ for methods). Blinding was not reported (NR for blinding). Overall rating = NR

Strakovsky et al. 2015

Risk of bias

HAWC