Pups were sacrificed on PND12 via decapitation and brains post-fixed overnight in 4% paraformaldehyde at 4 degrees C with mild shaking followed by an additional 24 hours with fesh 4% paraformaldehyde. All brains were then cruoprotected.

Microglia: only stated to use standard immunohistochemistry procedures. They cited Patisaul 2009, but Patisaul did not measure microglia. It was noted that Iba1 was used as the primary antibody because it is specific to microglia. Brain atlas was used to provide sections containing the dentate gyrus. Three morphological categories were quantified based on descriptions in Karperien et al., 2013. (+ for method). Counting was done using unbiased sterology using the optional fractionator probe of the Stereologer software. Mitigating the need for blinding. (+ for blinding). Overall +

Rebuli et al. 2016

Risk of bias

HAWC