Anxiety test: conducted on PND 56-61 using the elevated plus maze. Each animal was tested only once (no indication that order was random). Maze was cleaned between tests. Methods are well-established and criteria were provided, but tests were conducted in the alcove of the housing room to avoid transportation stress and there is not enough information provided to determine that this would not further stress the animals being housed and testing was conducted over several days. (+ for methods). Observers were blind to treatment (++ for blinding). Overall rating=+

Aggression test: conducted on PND121 using the resident-intruder paradigm. Scoring was based on cited methods. Criteria were mentioned. Methods are standard (+ for methods). Observers were blind to treatment (++ for blinding). Overall =+

Patisaul and Bateman 2008

Risk of bias

HAWC