F2 pup ultrasonic vocalization: Each pup was tested in isolation and away from the home-cage. Tests were initiated on PND2. Pup recordings were performed at the same times of day. Details of the method were provided. Pups were recorded for 3 minutes. Pups were stated to only be gone from their parents for 5 minutes. All pups within a litter were recorded for a given day before testing the next litter. However, the order of testing was randomized between treatments such that pups within the same litter were not always tests first or last on a given day. Once the pups were returned to the home cage, the pups were isolated again and returned to the audio-recording box and vocalizations were recorded for another 3 minutes. (+ for methods). Vocalizations were measured by MATLAB-based program. Experimenter was also stated to be blind to treatment when denoising and segmenting the calls. However, it was not noted that the research personnel handling the animals was blind to treatment. (+ for blinding). Overall =+

Johnson, Farrington et al. 2018

Risk of bias

HAWC