It was noted in the statistical methods that all analyses were conducted by experimenters blind to treatment group (++ for blinding).

LH measurements: LH was measured using the mouse reference preparation and anti-LH antibody. Sensitivity and interassay CV from duplicate samples of pooled samples was 3% (+ for methods). Overall=+

Estrous cyclicity: vaginal smears were used beginning at 5.5 months. Details were not provided on determinations for the different parts of the cycle, but details were provided on what was considered normal or extended (+ for methods). Overall=+.

Anti-Mullerian hormone assay (considered an indirect measure of ovarian reserve): was measured using a kit (+ for methods). Overall =+.

Acevedo et al. 2018

Risk of bias

HAWC