All samples were sent coded and analysed blind with decoding done for statistical analyses. For the real-time PCR controls had to be identified prior to running the tests so that controls could be included in each 96 well plate. However, even after decoding all samples were still blind to the operator handling the samples. (++ for blinding).

Serum Hormone measurements: Commercial kits were used for analysis with sensitivity provided and validation described. (++ for methods).

In Situ Hybridization: Methods are well-established and described. (++ for methods).

Cerebellar Histogenesis: Methods are well-established and described. (++ for methods).

Real-time PCR: Methods are well-established and described. (++ for methods).

Bansal and Zoeller 2019

Risk of bias

HAWC