Adult female Wistar rats (three months old, around 250–300 g body weight) were used. The animals (2 females and 1 male in a separate cage) were kept in standard laboratory conditions with 12 h light/dark periods at a temperature of 22 ± 2°C for breeding and supplied with dry rat food and drinking water ad libitum. The rats were acclimatized for one week prior to the commencement of the experiment. All experimental protocols were approved by the Ethics Committee of our institution as per CPCSEA No: 383/01/a/CPCSEA. After rat’s pregnancy was confirmed, from the first-day pregnancy, the females were segregated into the experimental groups and were provided fluoridated water (tap) at the rate of 20 ppm NaF.