The study was carried out on albino wistar rats (weighted about 250 - 300 g). Animals were housed in conventional condition at a temperature 24 ± 2 ºC with a relative humidity of 60 ± 5% and a 12-h / 12-h light / dark cycle and maintained as per ethical committee guidelines. Animals were allowed in animal house one week before starting the experiment. The rats were divided randomly to four experimental groups of 10 animals each.