Sprague-Dawley rats were obtained from the Center of Laboratory Animals of Guizhou Medical University (Guiyang, Guizhou, China). The pregnant rats were approximately 250 g in weight and singly housed under controlled temperature (20 ± 5 °C) and lighting (12 h light/12 h darkness) conditions and a relative humidity of 60 ± 2% with food and water ad libitum. The day that a vaginal plug was observed was defined as day 0 of gestation. The 16 pregnant rats were randomly divided into four groups. According to preliminary experimental results, the pregnant rats were exposed to 120 mg/l (1/10 LD50) NaF and/or 600 mg/l (1/10 LD50) of AlCl3 in their drinking water during pregnancy and for the first 21 days after giving birth. The experiment included one control group and 3 experimental groups, generated by adding NaF and AlCl3 to the tap water at the following concentrations: (0, 0), (120, 0), (0, 600), and (120, 600) mg/L, respectively. The groups were designated A, B, C, and D, respectively. From postnatal day (PND) 22 to PND90 (sexual maturity), the offspring rats were treated with the same concentrations of F and Al as the maternal rats through the same procedure.