Mammary gland development: Female offspring were sacrificed at PND30, 50, 110, and 180. Two hours before sacrifice, animals were injected with BrdU to determine proliferative index. This is a standard method (+ for methods).

Mammary gland lesions: Animals from the control were split to receive 25 mg/kg NMU (considered control) or 50 mg/kg NMU (considered positive control). BPA animals received 25 mg/kg NMU. NMU treatment occurred when the animals were 50 days old. Animals were sacrificed at PND110 or 180. Mammary glands were whole mounted. Lesions were removed and embedded in parafin for histological evaluation or immunohistochemistry (+ for methods).

Blinding or other methods to reduce bias were not reported (NR for blinding).

Durando et al. 2007

Risk of bias

HAWC