| Developmental GD8-16 |
Developmental |
Nitrofen was recrystallized from technical grade to a purity of 99.59% and administered in 0.2-mL corn oil by gavage to female 90-day-old pregnant Sprague-Dawley CD rats at doses of 0, 0.46, 1.39, 4.17, or 12.5 mg/kg-day on GDs 8−16. Dosing solutions were prepared based on the average weight of all rats on GD 7. Dams were weighed on GDs 7 and 17. Any dams that had not delivered by GD 24 were necropsied. Dead pups found on postnatal day (PND) 0 (the day of birth) were necropsied for cause of death. Numbers of pups per litter and litter weights were recorded on PNDs 0, 1, 2, and 6. Percentages of eyes open on PND 16 were calculated. |