Trivedi 2011
Risk of bias
Animal bioassay experiments
Name | Type | Comments |
---|---|---|
30-day oral | Subchronic (30-90 days) | The same eighty young adult inbred Swiss strain male albino mice (Mus musculus) employed in our previous laboratory work were used with approval by committee for the purpose of control and supervision of experiments for animals (Reg-167/1999/CPCSEA). Eighty g of black tea solids (Lipton Yellow Label of Hindustan Lever Limited, Mumbai, India) and 4 L of deionised water were used to produce 2% tea infusion, similar to the procedure we reported earlier.30 All the treatments were given orally for 30 days using a feeding tube attached to a hypodermic syringe (Table 1). After 30 days of treatment, the animals were sacrificed by cervical dislocation, brain of control and all treated groups of animals were quickly isolated and blotted free of blood. The CH (cerebral hemisphere), CB (cerebellum), and MO (medulla oblongata) regions of brain were separated carefully at low temperature and stored immediately in frozen condition to preserve enzyme activity until the following tests were performed. |