Adedara 2017a
| Data type(s) | Animal bioassay |
|---|---|
| Full citation | Adedara IA, Abolaji AO, Idris UF, Olabiyi BF, Onibiyo EM, Ojuade TD, Farombi EO. 2017. Neuroprotective influence of taurine on fluoride-induced biochemical and behavioral deficits in rats. Chem Biol Interact 261: 1-10. |
| Abstract | Epidemiological and experimental studies have demonstrated that excessive exposure to fluoride induced neurodevelopmental toxicity both in humans and animals. Taurine is a free intracellular beta-amino acid with antioxidant and neuroprotective properties. The present study investigated the neuroprotective mechanism of taurine by evaluating the biochemical and behavioral characteristics in rats exposed to sodium fluoride (NaF) singly in drinking water at 15 mg/L alone or orally co-administered by gavage with taurine at 100 and 200 mg/kg body weight for 45 consecutive days. Locomotor behavior was assessed using video-tracking software during a 10-min trial in a novel environment while the brain structures namely the hypothalamus, cerebrum and cerebellum of the rats were processed for biochemical determinations. Results showed that taurine administration prevented NaF-induced locomotor and motor deficits namely decrease in total distance travelled, total body rotation, maximum speed, absolute turn angle along with weak forelimb grip, increased incidence of fecal pellets and time of grooming, immobility and negative geotaxis. The taurine mediated enhancement of the exploratory profiles of NaF-exposed rats was supported by track and occupancy plot analyses. Moreover, taurine prevented NaF-induced increase in hydrogen peroxide and lipid peroxidation levels but increased acetylcholinesterase and the antioxidant enzymes activities in the hypothalamus, cerebrum and cerebellum of the rats. Collectively, taurine protected against NaF-induced neurotoxicity via mechanisms involving the restoration of acetylcholinesterase activity and antioxidant status with concomitant inhibition of lipid peroxidation in the brain of rats. |
| Literature review tags | Animal Study |
| COI reported | Authors report they have no COI |
| Funding source | This research was done without specific grant from any funding agency in the public, commercial, or not-for-profit sectors. |
| Study identifier | {Adedara, 2017 #710} |
| Author contacted? | true |
| Author contact details | Author was contacted in September 2017 to obtain information for RoB assessment. No response was received to email request for clarification. |
| Summary/extraction comments | Figures 1-2; Figure 3 qualitative only; Figures 4-6 Extract control and NaF alone groups, but make note in result comments if taurine had an effect on the results. |
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Animal bioassay experiments
| Name | Type | Comments |
|---|---|---|
| 45-day oral water | Subchronic (30-90 days) | Sixty adult male Wistar rats weighing between 120 g and 140 g obtained from the Department of Biochemistry, University of Ibadan, Ibadan were used for this study. The animals were housed in plastic cages placed in a well-ventilated vivarium and subjected to natural photoperiod of 12-hr light: 12-hr dark. They were fed with rat chow and given drinking water ad libitum for a week before the commencement of the experiment. All the animals received humane care according to the conditions stated in the ‘Guide for the Care and Use of Laboratory Animals’ prepared by the National Academy of Science (NAS) and published by the National Institute of Health. The experimental protocols were carried out after approval by the University of Ibadan Ethical Committee. |