Pregnant Wister albino rats were obtained from NCLAS, National Institute of Nutrition, Hyderabad, India. The pregnant rats were maintained under standard laboratory conditions in single rat polypropylene cages, exposed with 12 h light/dark cycle at room temperature 24 ± 2 C. The animals were provided standard diet and drinking water with ad libitum. The laboratory conditions were maintained as per the guidelines given by the CPCSEA (Committee for the Purpose of Control and Supervision on Experimental Animals) at Osmania University, Hyderabad, India.