Animal bioassay experiments

Name	Type	Comments
30-day rat drinking water	Subchronic (30-90 days)	The experiments were conducted on one-month-old adult male rats of the Wistar strain. The animals were bred at the Manipal University, Manipal, India. The animals were reared in polypropylene cages with paddy husk as bedding material. The animals were maintained at 12:12 hour light and dark cycle, with free access to food and water. Prior approval was obtained from the institutional animal ethics committee (IAEC/KMC/21/2009–2010). All the experiments were conducted according to the guidelines given by the CPCSEA Government of India.