| 4-week study |
Short-term (1-30 days) |
Four week-old C57/BL male mice from the Animal Center of the College of Medicine, Xi’an Jiaotong University were housed(26 cm × 18 cm × 13 cm) under a controlled 12-h/12-h light–darkcycle (lights on at 7:00 A.M.) at a room temperature of 22 ± 1◦C and 55 ± 5% humidity. Four mice were housed in each cage. Mice were given free access to water and food. Experiments were per-formed after 4 weeks of feeding; they were considered adults at 8weeks. The overall design scheme for behavioral tests is shown in Fig. 1. Before each test, the animals were placed in the laboratory for 30 min to be acclimated to the test environment. The experimental protocols were approved by the Xi’an Jiaotong University Labora-tory Animal Administration Committee. All efforts were made to minimize the number of animals used and their suffering. |