Young Wistar rats, weighing 50-60 g (18 days old) were used in this study. The rats were housed in a temperature-controlled room 22‑24°C with a 12:12 light: Dark cycle. Water and food were given ad libitum. All protocols described were reviewed and approved by the Local Institutional Committee for the Ethical Use of Animals and the laboratory was approved by Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India. (Approval no. #973/ac/06/CPCSEA).