| chronic drinking water |
Chronic (>90 days) |
Investigated effects of long-term
fluoride exposure on object recognition memory and
mRNA expressions of SNARE complex in the hippocampus of male mice. Animal husbandry: Sixty sexually matured male Kunming mice (each weighing 20–25 g) were purchased from the Experimental Animal Center of Shanxi Medical University. The mice were first habituated for 1 week in a housing facility before treatment…During the whole experimental period, animals had free access to food and water and were kept on their normal diets under standard temperature (22–25 °C), 12/12-h light/dark cycle, ventilation, and hygienic conditions. Throughout the whole experiment, fluoride in food was controlled at 8.40 mg/kg. This study protocol was approved by the Institutional Animal Care and Use Committee of Shanxi Agricultural University. |