| Reproductive study - allopathic doses |
Developmental |
NaF solution 0,25 %0 ; NaF solution 0,5 %0 ; NaF homeopathic remedy, 7CH dilution;
hematoxylin-eosine (HE) solution; After mating, gestating females were divided in 3 groups (16
animals/group) and were treated as follows: group I – 0.25 mg NaF, 0.25 %0 solution p.o.; group II – 0.5 mg NaF, 0.5 %0 solution p.o.; group III – distilled
water 0.1 mL/10 g-bw p.o.;
Administration of substances started from day 8 after mating, main
duration 14 ± 3 days (until litter deliverance). No substances were administered
further to females once litter delivered.
From the litters 14 groups were formed (generation 1 – noted F1)
treated according to table I description.
Upon sexual maturity, two pairs of each F1 group were randomly
chosen to breed next. This generation did not receive treatment and was noted
as generation 2 (F2).; male and female white mice, NMRI strain; Male and female laboratory mice NMRI strain were studied.
Females were housed together with males for a week for reproductive
purposes. |