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Developmental |
Healthy adult Wistar rats (180–200 g) were obtained from the
National Institute of Nutrition, Hyderabad. They were kept in polypropylene
cages, housed in a room at 22 ± 2 C on alternative 12 h
light–dark cycle, fed with standard chow diet (National Institute of
Nutrition), and provided with water ad libitum. After 1 week of
acclimation, one male and two females were placed together in
the cage for mating. The females were separated and moved to separate
cages after the appearance of the vaginal plug. All the experiments
were carried out in accordance with the guidelines set forth
by the Committee for the Purpose of Control and Supervision of
Experiments on Animals (CPCSEA), Ministry of Social Justice and
Empowerment, Government of India and have been approved by
the Institutional Animal Ethical Committee (IAEC) bearing number
NCOP/IAEC/approved/23/2011 dated 20.09.2011.
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