| Sprague-Dawley rats |
Chronic (>90 days) |
The fluoride level of the diet was low (14 ppm). Rats in the control group (n = 10) drank deionized water. The other groups drank water containing F added biweekly as NaF (75 or 150 ppm F; 150 ppm F for Sprague–Dawley and Lou/C rats, respectively). Treated water was administered for 104 and 195 days to Sprague–Dawley (n = 10 animals per concentration per test) and Lou/C (n = 12 animals per test) rats, respectively. Water consumption data were collected every day for the entire study. To validate the exposure of rats to F, plasma F levels were measured at the end of the experiment.; Male Sprague–Dawley (Charles River, St-Aubin-LesElbeuf, France) and Lou/C rats (Harlan, Gannat, France) weighing 80–100 g at the beginning of the experiment were used. They were randomly divided into four groups. They were housed in separated cages under standard laboratory conditions with free access to water and standard rat chow. |
| Lou C rats |
Chronic (>90 days) |
The fluoride level of the diet was low (14 ppm). Rats in the control group (n = 10) drank deionized water. The other groups drank water containing F added biweekly as NaF (75 or 150 ppm F; 150 ppm F for Sprague–Dawley and Lou/C rats, respectively). Treated water was administered for 104 and 195 days to Sprague–Dawley (n = 10 animals per concentration per test) and Lou/C (n = 12 animals per test) rats, respectively. Water consumption data were collected every day for the entire study. To validate the exposure of rats to F, plasma F levels were measured at the end of the experiment.; Male Sprague–Dawley (Charles River, St-Aubin-LesElbeuf, France) and Lou/C rats (Harlan, Gannat, France) weighing 80–100 g at the beginning of the experiment were used. They were randomly divided into four groups. They were housed in separated cages under standard laboratory conditions with free access to water and standard rat chow. |