| 30-day study |
Short-term (1-30 days) |
Mice were divided into seven groups of seven female
mice each. Group I served as control and mice were given access to
non-fluoridated water, and in addition 0.3% (w/v) CMC was orally administered
daily. Groups II and III received NaF 100 and 200 ppm, respectively, through
drinking water and in addition 0.3% (w/v) CMC was administered orally for both
the groups. Groups IV and V received NaF 100 ppm, and BM was co-administered
orally at the doses of 100 and 300 mg/kg, respectively. Groups VI and VII
received NaF 200 ppm, and BM was co-administered orally at the doses of 100 and
300 mg/kg, respectively.Behavioral tests were carried out on the 30th day of the study period. Before grouping the mice, they were all trained for 2 days in order to remain for 180 sec on a rod rotating at constant speed of 25 r/minutes; it took 3 trials for 2 days. On the day of the test, only the mice that were able to stay balanced on the rotating rod for 180 seconds were selected for the study. The behavioral tests carried out consisted of the akinesia, rotorod test, forced swim test, and open field test. |