| 1-2 week subcutaneous |
Short-term (1-30 days) |
Immunodeficient NOD/SCID gamma mice,
strain NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG; Jackson Laboratory, Bar Harbor,
ME), were maintained under pathogenfree conditions within the institutional
animal facility.21,22 Mice were injected subcutaneously (SC) with 3 × 105
luciferase (Luc2; Capital Biosciences, Rockville, MD)-labeled lung cancer H460
cells and 6 × 105 PM-exposed cells (1:2 ratio) suspended in 100 μL of ExtraCel
hydrogel (Advanced BioMatrix, San Diego, CA). Tumor growth of
luciferase-labeled cells was monitored weekly using IVIS bioimaging
(PerkinElmer, Waltham, MA). At the end of experiments, mice were euthanized,
and SC tumors were dissected and weighted. Metastasis of tumor cells to
neighbor tissues was analyzed by IVIS imaging after removal of primary SC
tumors |