Turner et al. 2013
Risk of bias
Animal bioassay experiments
Name | Type | Comments |
---|---|---|
C. elegans study | Other | We used C. elegans to investigate the toxicity and mechanism
of toxicity of environmental samples from the Mud River and several associated
tributaries (Boone County, Southwestern West Virginia). The Mud River flows
through the Hobet 21 surface mine from which it receives numerous discharges,
both directly and via its tributaries; the sampling sites have previously been
characterized with respect to water chemistry and show both conductivity in
excess of the proposed EPA benchmark of 300µs/cm-1 and selenium levels in
excess of EPA water quality criteria of 5µG Se L-1 [3]. Previous work has
suggested that increased levels of metals and total dissolved solids (TDS) may
be important stressors in MTR/VF systems [6]. We hypothesized that water and
sediment samples from putatively mining-impacted streams would affect the
growth of wild-type nematodes, and that mutant nematodes would be
differentially affected based on the composition of the stream water and
sediment pore water. Furthermore, we analyzed mutant strains that would help us
distinguish the contributions of metals or metalloids, osmotic stress and
oxidative stress to the toxicity of these samples (Table 1). NOTE FROM EXTRACTOR: MECHANISTIC DATA AVAILABLE IN SUPPLEMENTAL FIGURES BUT DATA NOT EXTRACTED |