| two-generation |
Reproductive |
Crl:CD(SD)IGS BR VAF/Plus® (Sprague–Dawley) rats were
supplied by Charles River Laboratory (Kingston, NY—male rats; and Raleigh,
NC—female rats). Rats were housed individually except during mating and
lactation. All cage sizes and housing conditions were in compliance with the
“Guide for the Care and Use of Laboratory Animals”. Rats were maintained on a
12:12 h light–dark cycle and fed ad libitum Certified Rodent Diet® #5002 (PMI
Nutrition International, St. Louis, MO). Water was provided ad libitum from individual
bottles and/or from an automatic watering access system. Bed-o’cobs® bedding
(Anderson’s Industrial Products, Maumee, OH) was used as the nesting material.
The study was conducted in an AAALAC accredited facility. |