Mouse: The research described in this study was conducted in a laboratory accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care International and the investigators complied with the regulations and standards of the Animal Welfare Act and adhered to the principles of the Guide for the Care and Use of Laboratory Animals.

The study design complied with the USEPA, OPPTS 870.7800: Immunotoxicity, Health Effects Test Guidelines (1998).
Male Crl:CD(SD)IGS BR rats and Crl:CD-1(ICR)BR mice were obtained from Charles River Laboratories, Inc. (Raleigh, NC). Animal rooms were targeted at a temperature of 22–24C and a relative humidity of 40–60%, and were artificially illuminated (fluorescent light) on a 12-h light/dark cycle. Throughout the test period, LLC Certified Rodent LabDiet 5002 (PMI Nutrition International, Inc., St Louis, MO) and tap water were available ad libitum. Prior to testing, rats and mice were evaluated by clinical observations and body weight determinations during a 4-day quarantine period to assure freedom from potential confounding variables.

Male Crl:CD-1(ICR)BR mice

HAWC