The acquisition and retention data subjected to two-way ANOVA with repeated measures revealed a significant difference between groups F(6, 35)=3.596; p<0.01 and sessions F(6, 210)=39.254; p<0.001. The latency data also revealed a significant difference between groups F(6, 35)=3.742; p<0.01 and sessions F(6, 210)=20.831; p<0.001. The third generation fluoride-treated rats of both doses took more sessions to learn a task (acquisition), performed poorly, and took more time to reach the goal area (latency) when compared with the second- and first-generation rats. Memory in terms of the number of errors committed during a single session of 30 trials showed no significant difference between the three generation fluoride-treated rats (Fig. 4).

Combined Wistar Albino rat (F1)

HAWC