Data to extract:

1) figure 1 data - note this should be in 4 separate experiments (1 week, 2, 3, and 4)

B:spleen: relative weight
C: lymphocyte proliferation (ConA)
D: spleen CD3+ cells - note "% total" in units
E: spleen CD8+ cells - note "% total" in units
F: spleen CD4+ cells - note "% total" in units
G: spleen CD3+ cells - note "cells normalized to control" in units
H: spleen CD8+ cells - note "cells normalized to control" in units
I: spleen CD4+ cells - note "cells normalized to control" in units

Qualitative description of gene profile and bioinformatics anlyses
1) The enrichment analysis showed ... MHCII-mediated immunity (enrichment factor 3.7), hematopoiesis (3.2) ... were also enriched, suggesting that these cellular processes were affected by PFOS
2) We then used Ingenuity tools to perform pathway analysis of the DEGs to gather all the information on the molecular mechanism of PFOS-induced immunotoxicity. A total of 501 genes were mapped in the Ingenuity knowledge base, and based on criteria (p < 0.05 and >5 genes in the pathways), 66 pathways were significantly enriched in the treatment group. ... Among them, the ones related to T-cell development and signaling are of particular biological significance in terms of the immunotoxicity of PFOS because T cells play an important role in cell-mediated immunity.

To further confirm the results of microarray analyses, we selected a few key genes involved in these pathways and used QRTPCR to quantify changes in levels of expression. These genes were determined to be related to: TCR signaling (CD3G and Themis), calcium ion regulation and signaling, including CAMK4 and CACNA1A, MAPK signaling (MAPK12 and MAP3K5), and cell cycle regulation (E2F1, CDC25A, CDK1, and CDKN1A). A similar trend in

was observed between QRT-PCR and the microarray data

Male BALBL/C mice

HAWC